Journal: Journal of radiation research

Article Title: Hypoxia expression in radiation-induced late rectal injury.

doi: 10.1269/jrr.07099

Figure Lengend Snippet: Fig. 4. Double immunofluorescence staining in fibrotic region of irradiated rectal tissue. (A), HIF-1α and VEGF; (B), HIF-1α and CD31. HIF-1α was labeled with red color; VEGF and CD31 were labeled with green color and Merge was shown by yellow color. Cont: unirradiated control mice. Magnification: × 100.

Article Snippet: Briefly, the slides were treated with rabbit anti-TGF-β1 polyclonal antibody (1:150, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit anti-HIF-1α polyclonal antibody (1:150, Santa Cruz Biotechnology), rabbit anti-VEGF polyclonal antibody (1:150, Santa Cruz Biotechnology) or rabbit anti-PECAM-1 (CD31) polyclonal antibody (1:150, Santa Cruz Biotechnology) as a primary antibody for overnight at 4°C.

Techniques: Double Immunofluorescence Staining, Irradiation, Labeling, Control

Journal: Glycobiology

Article Title: A polysaccharide, MDG-1, induces S1P1 and bFGF expression and augments survival and angiogenesis in the ischemic heart.

doi: 10.1093/glycob/cwp199

Figure Lengend Snippet: Fig. 7. MDG-1 protects myocardial cells from ischemia-induced damage and promotes angiogenesis in rats in vivo. The anti-myocardial ischemic effects of MDG-1 in vivo were assessed using a model of acute myocardial ischemia in rats as described in Materials and methods. (A) Representative photomicrographs of HE stained histological slides of the rat hearts are shown from the AMI, bFGF, and MDG-1 groups; the infarct areas are stained with amethyst color by HE. (C) Means ± SD of infarct size for five section determinations are shown under the figures (∗P < 0.05; #P < 0.01). (B) Photomicrography shows representative immunohistochemical CD31 staining of ischemic myocardium harvested at day 28. Dots (arrow) indicate capillaries. The images shown are representative of sections obtained from at least five heart samples. (D) Effect of MDG-1 on the mean number of capillaries per mm2 of the infarcted area (#P < 0.01).

Article Snippet: Briefly, all paraffin sections were incubated overnight at 4◦C with rabbit anti-mouse CD31 (1:100, Boster, Wuhan, Hubei province, China).

Techniques: In Vivo, Staining, Immunohistochemical staining

Journal: Viruses

Article Title: Circulating Plasma Exosomal Proteins of Either SHIV-Infected Rhesus Macaque or HIV-Infected Patient Indicates a Link to Neuropathogenesis

doi: 10.3390/v15030794

Figure Lengend Snippet: In vitro BBB permeability assays and the detection of PECAM-1 by immunofluorescence confocal microscopy in primary HBMVECs exposed to HIV+ Patient-Exo. ( a ) Schematic diagram of transwell migration-based BBB permeability in vitro assay. ( b ) Graph showing the BBB permeability efficiency of fluorescence labeled high molecular weight dextran (FITC-Dextran, 150 kDa) in the absence (black line) and presence (red, blue, and green lines) of cell monolayer. Cells without treatment (Cells only) or treated with normal human plasma exosomes (+CTL-Exo) were used as controls. The increased BBB permeability in cells treated with HIV-infected and cART-treated patient plasma exosomes (+Patient-Exo) is indicated by the red line. N = 4 experimental replicates were performed. ( c ) Representative immunofluorescent confocal microscope images of HBMVEC expressing PECAM-1 after 24 h treatment (10 µg/mL) with either hCTL-Exo or Patient-Exo. ( d ) The relative fluorescence intensity of PECAM-1 per view field was compared.

Article Snippet: Prior to staining, fixed cells were permeabilized with PBST (PBS with 0.1% Triton X-100) for 20 min. For staining using antibodies, cells were blocked for non-specific binding using PBS containing 5% donkey serum (Sigma-Aldrich Cat. No.: D9663) for 1 h at room temperature, followed by overnight incubation at 4 °C with a rabbit PECAM-1 (CD31) polyclonal antibody (BiCell Scientific Cat. No.: 01004) at a 1:200 dilution in PBS with 5% donkey serum.

Techniques: In Vitro, Permeability, Immunofluorescence, Confocal Microscopy, Migration, Fluorescence, Labeling, Molecular Weight, Infection, Microscopy, Expressing